|
KMID : 1237720220550020179
|
|
Anatomy & Cell Biology
2022 Volume.55 No. 2 p.179 ~ p.189
|
|
|
A study on the effect of JNJ-10397049 on proliferation and differentiation of neural precursor cells
|
|
Karami Neda
Azari Hassan
Rahimi Moosa
Aligholi Hadi
Kalantari Tahereh
|
|
|
Abstract
|
|
|
|
The orexin 2 receptor plays a central role in maintaining sleep and wakefulness. Recently, it has been shown that sleep and wakefulness orchestrate the proliferation and differentiation of oligodendrocytes. Here, we explored the role of a selective orexin 2 receptor antagonist (JNJ-10397049) in proliferation and differentiation of neural progenitor cells (NPCs). We evaluated the proliferation potential of NPCs after exposure to different concentrations of JNJ-10397049 by using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide and neurosphere assays. Moreover, the expression of differentiation markers was assessed by immunocytochemistry and real-time polymerase chain reaction. JNJ-10397049 significantly increased the proliferation of NPCs at lower concentrations. In addition, orexin 2 receptor antagonist facilitated progression of differentiation of NPCs towards oligodendroglial lineage by considerable expression of Olig2 and 2’,3’-cyclic-nucleotide 3’-phosphodiesterase as well as decreased expression of nestin marker. The results open a new avenue for future investigations in which the production of more oligodendrocytes from NPCs is needed.
|
|
|
KEYWORD
|
|
|
Orexins, Orexin receptors, JNJ 10397049, Oligodendroglia, Neural stem cells
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
 
|